The “in silico–in vitro–in vivo” funnel holds significant potential for identifying targets to control cellular processes in research and clinical applications. In this report, we describe a framework for identifying, selecting, and applying chemokines to direct retinal neuron migration in vivo within the adult mouse retina. To reach a broader audience, we demonstrate this phenomenon using dissociated mouse and human stem cell–derived retinal neurons, retinal organoids, and endogenously reprogrammed retinal neurons. Last, we show that only neurons that migrate into their proper lamina after transplantation express mature cell markers, indicating the importance of driving structural integration for neuron transplantation.
